Apoptosis Reagents and Kits
Apexbio Technology LLC Annexin V-APC/PI Apoptosis Kit 200tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (APC) can be used as a probe in combination with flow cytometry or fluorescence microscopy for detecting apoptosis and the staining process requires only 10-20 minutes Additionally this kit can be paired with the nuclear dye propidium iodide (PI) PI specifically binds to double-stranded DNA emitting red fluorescence upon binding but it cannot normally permeate intact cell membranes However PI can penetrate compromised cellular membranes in late-stage apoptotic and dead cells staining nuclei Therefore combined staining with Annexin V-APC and PI allows
Apexbio Technology LLC Annexin V-APC/SYTOX Green Apoptosis Kit 200tests
Annexin V-APC/SYTOX Green Apoptosis Kit is possible to differentiate cells at various stages of apoptosis and distinguish dead cells Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Moreover this kit contains SYTOX Green a nuclear probe that binds specifically to double-stranded DNA emitting green fluorescence SYTOX Green normally cannot permeate intact cell membranes It can penetrate the membranes of late-stage apoptotic cells or dead cells thus staining their nuclei
Abcam Human RHOA knockout HEK-293T cell lysate.
Human RHOA knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Apexbio Technology LLC Annexin V-APC/7-AAD Apoptosis Kit 200tests
Annexin V-APC/7-AAD Apoptosis Kit is possible to differentiate cells at various stages of apoptosis and distinguish dead cells Annexin V is a calcium-dependent phospholipid-binding protein which specifically binds to phosphatidylserine (PS) Under normal conditions PS is located on the inner surface of the cell membrane lipid bilayer but during early apoptosis PS translocates from the inner leaflet to the outer leaflet of the plasma membrane becoming exposed to the extracellular environment By using Annexin V-APC labeling apoptotic cells can be detected easily through flow cytometry or fluorescence microscopy within 15-30 minutes Additionally this kit contains the nuclear dye 7-AAD a fluorescent probe that specifically binds double-stranded DNA emitting red fluorescence Normally 7-AAD cannot permeate intact cell membranes However cells in the late apoptotic or necrotic stages exhibit compromised membrane integrity allowing 7-AAD to enter and stain the nuclei
Abcam Human PSMB8 (Proteasome 20S LMP7) knockout A549 cell lysate.
Human PSMB8 (Proteasome 20S LMP7) knockout A549 cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate.
Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human CD276 knockout HeLa cell lysate.
Human CD276 knockout HeLa cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human B2M (beta 2 Microglobulin) knockout Hep G2 cell lysate.
Human B2M (beta 2 Microglobulin) knockout Hep G2 cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Apexbio Technology LLC Apoptosis Inhibitor 54135-60-3 10mg
Apoptosis Inhibitor (CAS 54135-60-3) is a small molecule that modulates apoptotic processes by antagonizing pathways involved in caspase-3-mediated cell death It has been shown to suppress Fas ligand-induced cytotoxicity in human WC8 cells (IC50 67 g/ml) and etoposide-induced apoptosis in U937 cells (IC50 130 g/ml) as well as reduce caspase-3 activation in U937 cells (IC50 79 g/ml) The compound does not directly inhibit caspase-3 enzymatic activity in vitro indicating an upstream mechanism In vivo administration leads to decreased numbers of apoptotic neurons and reduced caspase-3 cells in neural and regenerative models Apoptosis Inhibitor is a valuable tool for studying caspase-dependent apoptosis and tissue regeneration
Cayman Chemical Caspase asy Buffer5X 5mL
Caspase-1 Assay Buffer (5X) has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Apexbio Technology LLC Annexin V-Cy5/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (Cy5) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence but normally cannot permeate intact cell membranes However DAPI can enter late-stage apoptotic or dead cells and stain their nuclei Simultaneous staining with Annexin V-Cy5 and DAPI allows differentiation of cells at various stages of apoptosis and cell death
Abcam Human H2AFY (mH2A1) knockout HEK-293T cell lysate.
Human H2AFY (mH2A1) knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human FYN knockout HEK-293T cell lysate.
Human FYN knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Human PARK7 (DJ1) knockout HEK-293T cell lysate.
Human PARK7 (DJ1) knockout HEK-293T cell lysate.
The product is subject to the following: Abcam Restricted Use Statement
Enzo Life Sciences SUPERFASLIGAND Protein (soluble) (human), (recombinant) (5 µg)
Fas ligand with improved stability providing significantly enhanced immune activation. Alternative name: FasL (oligomer), APO-1L (oligomer), CD95L (oligomer), CD178 (oligomer), TNFSF 6 (oligomer). MW: ~32kDa (nonglycosylated), ~35kDa (glycosylated). Concentration: 0.1mg/ml after reconstitution. Endotoxin content: <0.1EU/µg purified protein (LAL test; Associates of Cape Cod). Purity: ≥95% (SDS-PAGE). Formulation: Lyophilized. Contains PBS. Source: Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG-tag. Glycosylation of rhs SUPERFASLIGAND is similar to natural human FasL. Specificity: Binds to human, mouse and rat Fas (CD95; APO-1). Use/Stability: Stable for at least 6 months after receipt when stored at -20°C. UniProt: P48023. Application: ELISA. Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. Long Term Storage: -20°C